Pemeriksaan Hepatitis C dan Hepatitis B sebagai Parameter Infeksi Menular lewat Tranfusi Darah dengan Metode ELISA

Penulis

  • Gravinda Widyaswara STIKes Guna Bangsa Yogyakarta
  • Aulia Rahman STIKes Guna Bangsa Yogyakarta

Kata Kunci:

Hepatitis C, Hepatitis B, ELISA, IMLTD Test

Abstrak

Hepatitis can be caused by various diseases, including viral infections. Hepatitis C and Hepatitis B are two of the four parameters of Transmissible Infections Through Blood Transfusion (IMLTD) which until now have been used as a reference for screening tests for IMLTD at PMI UDD. Several methods that can be used for the IMLTD screening test include the Rapid Diagnostic Test (RDT), Enzyme-Linked Immunosorbent Assay (ELISA), Chemiluminescence Immuno Assay (ChLIA), and Nucleic Acid Amplification Test (NAT). ELISA is the gold standard for IMLTD examination, especially Hepatitis C and Hepatitis B. In addition, ELISA has a specificity of 98% in the IMLTD screening test. This study aims to determine the results of Hepatitis C and Hepatitis B examinations using the ELISA method for students from batch 2021 of the TBD STIKes Guna Bangsa Yogyakarta Study Program. This type of research is experimental using a cross sectional study design. A total of 2 blood samples were obtained by taking the cubital vein. The results of the examination using the ELISA method can be seen by calculating the absorption value, cut-off value, and negative control value. From the research conducted, it was obtained valid negative results with a value of <1. The absorption value of sample 1 is 0.5 and sample 2 is 0.57. Two samples had valid non-reactive results for HCV and HBsAg examinations. From these results, it can be concluded that the two respondents who were samples were eligible to donate blood at UDD PMI.

Unduhan

Diterbitkan

2024-02-06